Coding

Part:BBa_K2616002:Design

Designed by: Finn Stirling and iGEM Pasteur Paris   Group: iGEM18_Pasteur_Paris   (2018-09-05)


Temperature Kill Switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 802


Design Notes

Codons were optimized for E. coli and a part of the original sequence was deleted.


Source

References

Rational Design of Evolutionarily Stable Microbial Kill Switches. Stirling F, Bitzan L, O'Keefe S, Redfield E, Oliver JWK, Way J, Silver PA.